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Nutrient Agar Culture Medium (Biochemical Reagent)

Product description

Product Name: Nutrient Agar (NA) Dehydrated Culture Medium

Key Features and Specifications

Feature CategorySpecification
TypeGeneral-Purpose, Non-Selective Basal Medium
FunctionCultivation and Enumeration of a wide variety of non-fastidious microorganisms (Bacteria and Yeast)
FormulationContains Peptone (source of nitrogen and amino acids), Beef Extract (vitamins, minerals), and Agar (solidifying agent)
FormatDehydrated Powder (Ready-to-use after hydration and sterilization)
pH (Final)Typically 7.4  0.2 (Optimal for most bacteria)
Sterilization MethodAutoclavable (Typically 121°C for 15 minutes)
Key AdvantageBroad-Spectrum Support for standard laboratory procedures; excellent for routine plating and maintenance.

Product Summary

Nutrient Agar (NA) is one of the most fundamental and universally used culture media in the Biochemical / Life Sciences and microbiology sectors. It is a basal medium, meaning it provides the basic nutritional requirements necessary to support the growth of a broad spectrum of non-fastidious (non-picky) microorganisms.

It’s simple formulation—primarily water, a protein digest source (peptone), beef extract, and agar—makes it the standard choice for routine subculturing, plate counting, and the storage of bacterial strains. It is a non-selective medium, allowing almost all bacteria present in a sample to grow, making it ideal for initial screening and enumeration studies.

Primary Applications

  1. Microbiology & Bacteriology:

    • General Cultivation: Used for the routine growth and maintenance of common bacterial strains (e.g., E. coliB. subtilis).

    • Enumeration/Plate Counting: Essential for determining the total number of viable microorganisms in a sample (e.g., food, water, air) using the standard plate count method.

    • Sample Isolation: Initial streaking of environmental, clinical, or food samples to obtain isolated colonies for further characterization.

  2. Quality Control (QC):

    • Sterility Testing: Used as a reference medium to confirm the sterility of equipment, media, or products where microbial contamination is a concern.

  3. Educational & Training Laboratories:

    • Due to its broad applicability and simple preparation, Nutrient Agar is the medium of choice for introductory microbiology experiments and student training.

Preparation and Use

  • Convenience: Supplied as a dehydrated powder, requiring only the addition of purified water and sterilization by autoclaving before being poured into Petri dishes or dispensed into tubes.

  • Storage: Dehydrated media should be stored in a cool, dark, and dry place to maximize shelf life. Poured plates should be stored refrigerated (2-8°C) to maintain moisture.

  • Customization: Nutrient Agar can be supplemented with specific additives (e.g., blood, specialized sugars, or selective agents) to create enriched or selective media for more specialized microbial studies.

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